MVL Medium

[所属分类:培养基配方] [发布时间:2021-6-11] [发布人:网站管理员2] [阅读次数:] [返回]

MVL Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Beef extract...................................................................................2.0g
Glucose .........................................................................................2.0g
Mineral solution 1....................................................................75.0mL
Mineral solution 2....................................................................75.0mL
Na 2 CO 3  (8.0% solution)..........................................................50.0mL
Hemin (0.07% solution)...........................................................10.0mL
L- Cysteine·HCl·H 2 O (3.0% solution).......................................10.0mL
Resazurin (0.1% solution)..........................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Mineral Solution 1:
Composition per 100.0.mL:
K 2 HPO 4 .........................................................................................0.6g
Preparation of Mineral Solution 1: Add K 2 HPO 4 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly.
Mineral Solution 2:
Composition per 100.0mL:
(NH 4 ) 2 SO 4 ....................................................................................1.2g
NaCl..............................................................................................1.2g
KH 2 PO 4 .........................................................................................0.6g
MgSO 4 ·7H 2 O..............................................................................0.12g
CaCl 2 ·2H 2 O ................................................................................0.12g
Preparation of Mineral Solution 2: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Prepare and dispense medium under
100% CO 2 . Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Anaerobically dis-
tribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Tonsillophilus suis.