MVTY Medium

[所属分类:培养基配方] [发布时间:2021-6-11] [发布人:网站管理员2] [阅读次数:] [返回]

MVTY Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar.....................................................................................7.0g
Yeast extract..................................................................................2.0g
Pancreatic digest of casein............................................................2.0g
L -Cysteine·HCl·H 2 O.....................................................................1.0g
Salts A....................................................................................200.0mL
Salts B....................................................................................200.0mL
NaHCO 3 solution...................................................................100.0mL
Glucose (10% solution) ...........................................................20.0mL
Resazurin (0.1% solution) .........................................................1.0mL
n-Butyric acid............................................................................0.4mL
Isobutyric acid ...........................................................................0.2mL
DL -2-Methylbutyric acid............................................................0.2mL
n-Valeric acid.............................................................................0.2mL
Isovaleric acid............................................................................0.2mL
pH 6.9 ± 0.2 at 25°C
Salts A:
Composition per liter:
CaCl 2 ...........................................................................................0.45g
MgSO 4 ........................................................................................0.45g
Preparation of Salts A:  Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly.
Salts B:
Composition per liter:
NaCl..............................................................................................4.5g
(NH 4 ) 2 SO 4 ....................................................................................4.5g
KH 2 PO 4 .......................................................................................2.25g
K 2 HPO 4 .......................................................................................2.25g
Preparation of Salts B: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly.
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Separately autoclave the glucose solu-
tion, for 15 min at 15 psi pressure–121°C. Prepare and dispense the
basal salts A and B under 100% CO 2 . Add the agar, yeast extract, pan-
creatic digest of casein,  L -cysteine·HCl·H 2 O, salts A, salts B, and resa-
zurin to distilled/deionized water and bring volume to 478.0mL. Mix
thoroughly. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Add filter sterilized n-butyric acid, isobutyric ac-
id,  DL -2-methylbutyric acid, n-valeric acid, isovaleric acid, sterile
NaHCO 3 solution, and sterile glucose solution. Mix thoroughly. Asep-
tically and anaerobically distribute into sterile tubes.
Use:  For the cultivation and maintenance of Treponema saccharophi-
lum.