MWY Medium (Wadowsky and Yee Medium, Modified)

[所属分类:培养基配方] [发布时间:2021-6-11] [发布人:网站管理员2] [阅读次数:] [返回]

MWY Medium
(Wadowsky and Yee Medium, Modified)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................13.0g
Yeast extract................................................................................10.0g
Glycine..........................................................................................3.0g
ACES buffer (2-[(2-amino-2-oxoethyl)-
amino]-ethane sulfonic acid)..................................................2.0g
Charcoal, activated........................................................................2.0g
α-Ketoglutarate.............................................................................0.2g
Fe 4 (P 2 O 7 ) 3 ·9H 2 O.........................................................................0.05g
Bromcresol Purple ......................................................................0.01g
Bromcresol Blue.........................................................................0.01g
Antibiotic inhibitor ..................................................................10.0mL
L- Cysteine·HCl·H 2 O solution...................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Antibiotic Inhibitor:
Composition per 10.0mL:
Anisomycin.................................................................................0.16g
Cefamandole..............................................................................4.0mg
Vancomycin ...............................................................................1.0mg
Polymyxin B........................................................................130,000U
Preparation of Antibiotic Inhibitor: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
L - Cysteine·HCl·H 2 O Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H 2 O....................................................................0.08g
Preparation of  L -Cysteine·HCl·H 2 O Solution: Add  L- cyste-
ine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except  L -cysteine solu-
tion and antibiotic inhibitor, to distilled/deionized water and bring vol-
ume to 980.0mL. Mix thoroughly. Adjust medium to pH 6.9 with 1N
KOH. Heat gently and bring to boiling for 1 min. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°–55°C. Add 10.0mL of the sterile
L- cysteine·HCl·H 2 O solution and 10.0mL of the sterile antibiotic solu-
tion. Mix thoroughly. Pour into sterile Petri dishes with constant agita-
tion to keep charcoal in suspension.
Use: For the selective isolation and cultivation of Legionella pneumo-
phila and other Legionella species.