MY Agar

[所属分类:培养基配方] [发布时间:2021-6-11] [发布人:网站管理员2] [阅读次数:] [返回]

MY Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Sodium acetate..............................................................................0.1g
Yeast extract..................................................................................0.1g
Pancreatic digest of gelatin.........................................................0.06g
Beef extract.................................................................................0.04g
Basal medium ...............................................................................1.0L
Sodium sulfide solution.............................................................3.0mL
pH 7.0–7.5 at 25°C
Basal Medium:
Composition per liter:
CaSO 4 ·2H 2 O (saturated solution)............................................20.0mL
NH 4 Cl (4% solution) .................................................................5.0mL
Trace elements solution.............................................................5.0mL
K 2 HPO 4 (1% solution)...............................................................1.0mL
MgSO 4 ·7H 2 O (1% solution)......................................................1.0mL
Preparation of Basal Medium: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
Ferrous EDTA solution............................................................20.0mL
ZnSO 4 ·7H 2 O (0.1% solution)..................................................10.0mL
MnSO 4 ·4H 2 O (0.02% solution)...............................................10.0mL
CuSO 4 ·5H 2 O (0.00005% solution)..........................................10.0mL
H 3 BO 3 (0.1% solution)............................................................10.0mL
Co(NO 3 ) 2 or CoCl 2 ·6H 2 O (0.01% solution)............................10.0mL
Na 2 MoO 4 ·2H 2 O (0.01% solution)...........................................10.0mL
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Ferrous EDTA Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................7.0g
EDTA............................................................................................2.0g
HCl, concentrated......................................................................1.0mL
Preparation of Ferrous EDTA Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Sodium Sulfide Solution:
Composition per 10.0mL:
Na 2 S·9H 2 0.....................................................................................1.0g
Preparation of Sodium Sulfide Solution: Add Na 2 S·9H 2 0 to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Prepare freshly.
Preparation of Medium: Add sodium acetate, beef extract, pancre-
atic digest of gelatin, yeast extract, and agar to 1.0L of basal medium.
Mix thoroughly. Adjust pH to 7.0–7.5. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 3.0mL of sterile sodium sulfide solution immediately
prior to dispensing. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile screw-capped tubes.
Use: For the isolation and cultivation of Beggiatoa species and myx-
otrophic strains of Thiothrix species from water and environmental
sources.