Mycoplasma Agar (ATCC Medium 1435)

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Agar
(ATCC Medium 1435)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 930.0mL:

Agar, noninhibitory to mycoplasmas..........................................10.0g
Glucose .........................................................................................1.0g
Nicotinamide adenine dinucleotide ..............................................0.1g
PPLO broth without Crystal Violet........................................680.0mL
Swine serum (56°C, 30 min)..................................................150.0mL
Yeast extract (25% w/v solution)...........................................100.0mL
pH 7.8 ± 0.2 at 25°C
PPLO Broth without Crystal Violet:
Composition per 680.0mL:
Beef heart, infusion from............................................................11.3g
Peptone........................................................................................2.28g
NaCl............................................................................................1.13g
Source: PPLO broth without Crystal Violet is available as a premixed
powder from BD Diagnostic Systems.
Preparation of PPLO Broth without Crystal Violet:  Add
components and agar to distilled/deionized water and bring volume to
680.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Mix glucose, nicotinamide adenine dinu-
cleotide, swine serum, and fresh yeast extract solution. Mix thorough-
ly. Heat to 56°C. Add to cooled, sterile PPLO broth without Crystal
Violet. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use:  For the cultivation and maintenance of Mycoplasma anseris and
Mycoplasma lipofaciens.