Mycoplasma Agar Base (PPLO Agar Base)

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Agar Base
(PPLO Agar Base)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1300.0mL:

Agar ............................................................................................14.0g
Pancreatic digest of casein............................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, solids from infusion....................................................2.0g
Horse serum...........................................................................260.0mL
Fresh yeast extract solution......................................................65.0mL
pH 7.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium:  Add components, except horse serum
and fresh yeast extract, to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. To each 75.0mL of cooled, sterile basal medium, add
20.0mL of sterile horse serum and 5.0mL of special yeast extract. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the preparation of media for the cultivation of Mycoplasma.