Mycoplasma Agar with Increased Selectivity

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Agar with Increased Selectivity

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1300.0mL:

Agar............................................................................................14.0g
Pancreatic digest of casein............................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, solids from infusion....................................................2.0g
Thallous acetate............................................................................0.7g
Penicillin................................................................................ 70,000U
Horse serum...........................................................................260.0mL
Fresh yeast extract solution .....................................................65.0mL
pH 7.8 ± 0.2 at 25°C
Caution: Thallous acetate is a poison.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium:  Add components—except horse serum,
special yeast extract, thallous acetate, and penicillin—to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
and bring to boiling. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C. To each 70.0mL of
cooled, sterile basal medium, add 20.0mL of sterile horse serum,
10.0mL of special yeast extract, 50.0mg of thallous acetate, and 5000U
of penicillin. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the preparation of media for the cultivation of Mycoplasma
species.