Mycoplasma Broth

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Basal medium ........................................................................700.0mL
Horse serum...........................................................................200.0mL
Fresh yeast extract solution ...................................................100.0mL
pH 7.5–7.8 at 25°C
Basal Medium:
Composition per 700.0mL:
Sorbitol........................................................................................50.0g
Beef heart, solids from infusion..................................................16.2g
Peptone........................................................................................3.26g
NaCl............................................................................................1.62g
Fructose.........................................................................................1.0g
Glucose .........................................................................................1.0g
Sucrose..........................................................................................1.0g
Pancreatic digest of casein............................................................1.0g
Preparation of Basal Medium:  Add components to distilled/de-
ionized water and bring volume to 700.0mL. Mix thoroughly. Adjust
pH to 7.5–7.8. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium: Filter sterilize horse serum and fresh
yeast extract solution. Aseptically add to cooled, sterile basal medium.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Mycoplasma mycoides,
Spiroplasma apis, Spiroplasma citri, and Spiroplasma melliferum.