Mycoplasma Broth

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 950.0mL:

Glucose .........................................................................................1.0g
Nicotinamide adenine dinucleotide ..............................................0.1g
PPLO broth without Crystal Violet........................................680.0mL
Swine serum (56°C, 30 min)..................................................150.0mL
Fresh yeast extract solution....................................................100.0mL
Phenol Red (0.1% w/v solution)..............................................20.0mL
pH 7.8 ± 0.2 at 25°C
PPLO Broth without Crystal Violet:
Composition per 680.0mL:
Beef heart, solids from infusion..................................................11.3g
Peptone........................................................................................2.28g
NaCl............................................................................................1.13g
Source: PPLO broth without Crystal Violet is available as a premixed
powder from BD Diagnostic Systems.
Preparation of PPLO Broth without Crystal Violet:  Add
components to distilled/deionized water and bring volume to 680.0mL.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 56°C.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium: Mix glucose, nicotinamide adenine dinu-
cleotide, swine serum, fresh yeast extract solution, and Phenol Red.
Mix thoroughly. Heat to 56°C. Add to cooled, sterile PPLO broth with-
out Crystal Violet. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use:  For the cultivation and maintenance of Mycoplasma anseris and
Mycoplasma lipofaciens.