Mycoplasma Broth Base, Frey with Horse Serum

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Broth Base, Frey with Horse Serum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Pancreatic digest...........................................................................7.5g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................2.5g
Na 2 HPO 4 .......................................................................................1.6g
KCl................................................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.2g
KH 2 PO 4 .........................................................................................0.1g
Horse serum...........................................................................100.0mL
pH 7.7 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°C. Add sterile, heat-inactivated horse serum.
Mix thoroughly. Aseptically distribute into sterile tubes.
Use: For the cultivation of avian mycoplasmas.