Mycoplasma Broth, Supplemented

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Broth, Supplemented

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, solids from infusion....................................................2.0g
Horse serum...........................................................................260.0mL
Fresh yeast extract solution .....................................................65.0mL
pH 7.8 ± 0.2 at 25°C
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium: Add components, except horse serum
and fresh yeast extract solution, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. To each 75.0mL of cooled, sterile basal medium,
add 20.0mL of sterile horse serum and 5.0mL of fresh yeast extract so-
lution. Mix thoroughly. Aseptically distribute into sterile tubes.
Use:  For the isolation and cultivation of Mycoplasma species.