Mycoplasma Broth with Supplement G

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Broth with Supplement G

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Bacteriological peptone..............................................................10.0g
Beef extract.................................................................................10.0g
NaCl..............................................................................................5.0g
Special mineral supplement, Oxoid Unipath................................0.5g
Mycoplasma supplement G....................................................250.0mL
pH 7.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Mycoplasma Supplement G:
Composition per 20.0mL:
Thallous acetate .......................................................................25.0mg
Horse serum.............................................................................20.0mL
Yeast extract (25% solution)....................................................10.0mL
Penicillin................................................................................20,000U
Preparation of Mycoplasma Supplement G: Add components
to distilled/deionized water and bring volume to 20.0mL. Mix thor-
oughly. Filter sterilize.
Caution: Thallous acetate is a poison.
Preparation of Medium: Add components, except Mycoplasma
supplement G, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Distribute into flasks
in 80.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. Aseptically add 20.0mL of sterile Mycoplasma supple-
ment G to each 80.0mL of basal medium. Mix thoroughly.
Use: For the growth of Mycoplasma species.