Mycoplasma Broth with Supplement P

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Broth with Supplement P

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Bacteriological peptone ..............................................................10.0g
Beef extract.................................................................................10.0g
NaCl..............................................................................................5.0g
Special mineral supplement, Oxoid Unipath................................0.5g
Mycoplasma supplement P ....................................................250.0mL
pH 7.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Mycoplasma Supplement P:
Composition per 20.0mL:
Glucose .........................................................................................0.3g
Mycoplasma broth base............................................................0.145g
Thallous acetate .........................................................................8.0mg
Phenol Red.................................................................................1.2mg
Methylene Blue chloride............................................................0.3mg
Penicillin................................................................................12,000U
Horse serum...............................................................................6.0mL
Yeast extract (25% solution)......................................................3.0mL
Preparation of Mycoplasma Supplement P: Add components to
distilled/deionized water and bring volume to 20.0mL. Mix thorough-
ly. Filter sterilize.
Caution: Thallous acetate is a poison.
Preparation of Medium: Add components, except Mycoplasma
supplement P, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Distribute into bot-
tles in 1.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to room temperature. Aseptically add 2.0mL of sterile Mycoplas-
ma supplement P to each bottle.
Use: For the cultivation of Mycoplasma species.