Mycoplasma Broth with 10% Swine Serum

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Broth with 10% Swine Serum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................5.6g
NaCl..............................................................................................4.0g
Yeast extract..................................................................................2.6g
Beef extract...................................................................................2.4g
Beef heart, solids from infusion....................................................1.6g
Swine serum, heat inactivated ...............................................100.0mL
Fresh yeast extract solution ...................................................100.0mL
pH 7.8 ± 0.2 at 25°C
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium: Add components, except swine serum and
fresh yeast extract solution, to distilled/deionized water and bring volume
to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°C. Add sterile swine se-
rum and fresh yeast extract solution. Mix thoroughly. Aseptically distrib-
ute into sterile tubes.
Use:  For the cultivation and maintenance of Mycoplasma columbinum
and Mycoplasma columborale.