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Mycoplasma HiVeg Broth Base with Crystal Violet and Tellurite (PPLO HiVeg Broth Base with CV)
[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]
Mycoplasma HiVeg Broth Base
with Crystal Violet and Tellurite
(PPLO HiVeg Broth Base with CV)
Plant infusion................................................................................6.0g
NaCl..............................................................................................5.0g
Crystal Violet..............................................................................0.01g
Chapman tellurite solution.......................................................2.85mL
Ascitic fluid............................................................................250.0mL
pH 7.8 ± 0.2 at 25°C
Source: This medium, without tellurite, is available as a premixed
powder from HiMedia.
Chapman Tellurite Solution:
Composition per 100.0mL:
K 2 TeO 3 ..........................................................................................1.0g
Preparation of Chapman Tellurite Solution: Add K 2 TeO 3 to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components, except ascitic fluid and
Chapman tellurite solution, to distilled/deionized water and bring vol-
ume to 747.15mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to less than 37°C. Aseptically add sterile ascitic
fluid and 2.85mL of Chapman tellurite solution. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use: For the isolation of Mycoplasma species from clinical specimens.
with Crystal Violet and Tellurite
(PPLO HiVeg Broth Base with CV)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Plant peptone ..............................................................................10.0gPlant infusion................................................................................6.0g
NaCl..............................................................................................5.0g
Crystal Violet..............................................................................0.01g
Chapman tellurite solution.......................................................2.85mL
Ascitic fluid............................................................................250.0mL
pH 7.8 ± 0.2 at 25°C
Source: This medium, without tellurite, is available as a premixed
powder from HiMedia.
Chapman Tellurite Solution:
Composition per 100.0mL:
K 2 TeO 3 ..........................................................................................1.0g
Preparation of Chapman Tellurite Solution: Add K 2 TeO 3 to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components, except ascitic fluid and
Chapman tellurite solution, to distilled/deionized water and bring vol-
ume to 747.15mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to less than 37°C. Aseptically add sterile ascitic
fluid and 2.85mL of Chapman tellurite solution. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use: For the isolation of Mycoplasma species from clinical specimens.
