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Mycoplasma HiVeg Broth Base without Crystal Violet and with Ascitic Fluid (PPLO HiVeg Broth Base without CV)

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma HiVeg Broth Base
without Crystal Violet and with Ascitic Fluid
(PPLO HiVeg Broth Base without CV)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone...............................................................................10.0g
Plant infusion................................................................................6.0g
NaCl..............................................................................................5.0g
Ascitic fluid............................................................................250.0mL
pH 7.8 ± 0.2 at 25°C
Source: This medium, without ascitic fluid, is available as a premixed
powder from HiMedia.
Preparation of Medium: Add components, except ascitic fluid, to
distilled/deionized water and bring volume to 750.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to less than
37°C. Aseptically add sterile ascitic fluid. If desired, 0.5g of thallium
acetate or 100,000U of penicillin may be added for a more selective
medium. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the enrichment of pleuro-pneumonia-like organisms (PPLOs)
and Mycoplasma species from clinical specimens.

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Mycoplasma HiVeg Broth Base without Crystal Violet and with Ascitic Fluid (PPLO HiVeg Broth Base without CV)