Mycoplasma Horse Serum Broth (ATCC Medium 1959)

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Horse Serum Broth
(ATCC Medium 1959)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Mycoplasma broth base.........................................................660.0mL

Horse serum...........................................................................200.0mL
Fresh yeast extract solution....................................................100.0mL
Phenol Red (0.1%)...................................................................20.0mL
Glucose solution ......................................................................10.0mL
NaOH (1N solution).................................................................6.25mL
Arginine solution .......................................................................5.0mL
Mycoplasma Broth Base:
Composition per liter:
Pancreatic digest of casein............................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, solids from infusion....................................................2.0g
Preparation of Mycoplasma Broth Base: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Glucose Solution:
Composition per 10.0mL:
Glucose.........................................................................................5.0g
Preparation of Glucose Solution : Add glucose to 10.0mL of dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Arginine Solution:
Composition per 10.0mL:
L -Arginine.....................................................................................4.2g
Preparation of Arginine Solution : Add arginine to 10.0mL of
distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Combine 660.0mL Mycoplasma broth
base, 20.0mL Phenol Red, and 6.25mL 1N NaOH. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Asepti-
cally add 5.0mL sterile arginine solution, 100.0mL sterile fresh yeast
extract solution, 10.0mL sterile glucose solution, and 200.0mL filter
sterilized horse serum. Mix thoroughly. Aseptically distribute into ster-
ile tubes or flasks.
Use: For the preparation of media for the cultivation of Mycoplasma
spp.