Mycoplasma Liquid Medium

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Liquid Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1004.0mL:

Arginine........................................................................................1.0g
Glucose.........................................................................................1.0g
L- Cysteine·HCl·H 2 O......................................................................1.0g
Mycoplasma broth base .........................................................850.0mL
Horse serum, not inactivated .................................................100.0mL
Fresh yeast extract (25% solution) ..........................................50.0mL
Phenol Red (1.0% solution).......................................................2.0mL
DNA calf thymus solution.........................................................2.0mL
pH 7.8 ± 0.2 at 25°C
Mycoplasma Broth Base:
Composition per 850.0mL:
Pancreatic digest of casein............................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, solids from infusion....................................................2.0g
Preparation of Mycoplasma Broth Base: Add components to
distilled/deionized water and bring volume to 850.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
DNA Calf Thymus Solution:
Composition per 10.0mL:
DNA calf thymus..........................................................................1.0g
Preparation of DNA Calf Thymus Solution: Add DNA calf
thymus to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Combine components, except Mycoplasma
broth base and DNA calf thymus solution, and mix thoroughly. Filter ster-
ilize through a 0.2μm membrane. Add sterile solution to 850.0mL of
cooled, sterile Mycoplasma broth base. Aseptically add 2.0mL of sterile
DNA calf thymus solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use:  For the cultivation and maintenance of Mycoplasma lipophilum
and Mycoplasma species.