Mycoplasma Medium

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Heart infusion broth....................................................................25.0g
Mucin, bacteriological grade ........................................................5.0g
Agar, purified (optional)...............................................................7.0g
Hemoglobin ..................................................................................2.0g
Turkey serum, sterile inactivated...........................................100.0mL
pH 7.8 ± 0.2 at 25°C
Preparation of Medium: Add components, except turkey serum and
agar, to 850.0mL distilled water. Adjust pH to 7.8. Heat mixture at 93°–
95°C for 30 min in a water bath. Restore to original volume. Add 0.5% di-
atomaceous earth. Mix thoroughly. Filter through Whatman GFA (glass fi-
ber paper) in Buchner filter. Clarify using 0.45μm Millipore filter. Add
15% inactivated turkey serum. Sterilize using S3 (0.1μm) Seitz filter. Use
positive pressure. For solid medium : Prepare 42.5mL of double-strength
broth and 42.5mL of distilled water containing 0.7g of purified agar. Ster-
ilize the solutions separately and combine aseptically at 56°C with 15.0mL
of sterile inactivated turkey serum for a final volume of 150.0mL.
Use: For the cultivation and maintenance of Mycoplasma hyosyn-
oviae.