Mycoplasma Medium (CIP Medium 89) (DSMZ Medium 1080)

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma Medium
(CIP Medium 89)
(DSMZ Medium 1080)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein ...........................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, infusion from (solids).................................................2.0g
Selective supplement solution ...............................................210.0mL
Yeast extract solution.............................................................100.0mL
Phenol Red solution.................................................................20.0mL
Yeast Extract Solution :
Composition per 100.0mL:
Yeast extract................................................................................25.0g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C.
Phenol Red Solution :
Composition per 100.0mL:
Phenol Red....................................................................................1.0g
Preparation of Phenol Red Solution:  Add Phenol Red to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C.
Selective Supplement Solution:
Composition per 210.0mL:
Ampicillin.....................................................................................1.0g
Horse serum...........................................................................200.0mL
Arginine solution.....................................................................10.0mL
Arginine Solution :
Composition per 100.0mL:
L -Arginine...................................................................................50.0g
Preparation of  Arginine  Solution:  Add  L -arginine to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Adjust
pH to 7.0.
Preparation of Selective Supplement Solution: Add ampicillin
to 10.0mL arginine solution. Add horse serum. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except yeast extract,
Phenol Red, and selective supplement solutions, to distilled/deionized
water and bring volume to 670.0mL. Mix thoroughly. Adjust pH to 7.2.
Distribute into tubes or flasks. Gently heat while stirring and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature. Aseptically add yeast extract, Phenol Red, and selective
supplement solutions. Mix thoroughly. Aseptically distribute into tubes
or flasks.
Use: For the cultivation of Mycoplasma spp.