Mycoplasma pneumoniae Isolation Medium

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasma pneumoniae Isolation Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1200.0mL:

Beef heart for infusion................................................................50.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Water......................................................................................900.0mL
Yeast extract solution.............................................................100.0mL
α-Gamma horse serum, unheated..........................................200.0mL
pH 7.6–7.8 at 25°C
Yeast Extract Solution:
Composition per 10.0mL:
Yeast, active, dry, Baker’s.........................................................250.0g
Preparation of Yeast Extract Solution: Add yeast to 1.0L of dis-
tilled/deionized water. Mix thoroughly. Gently heat and bring to boil-
ing. Filter through Whatman #2 filter paper. Adjust the pH of the
filtrate to 8.0 with NaOH. Distribute into tubes in 10.0mL volumes.
Autoclave for 15 min at 15 psi pressure–121°C. Store at −20°C.
Preparation of Medium: Add components, except yeast extract so-
lution and α-gamma horse serum, to distilled/deionized water and
bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add sterile yeast extract solution and α-gamma horse
serum. Mix thoroughly. Aseptically distribute into sterile tubes.
Use: For the isolation and cultivation of Mycoplasma pneumoniae.