Mycoplasmal Agar

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Mycoplasmal Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Papaic digest of soy meal ...........................................................20.0g
Agarose.......................................................................................10.0g
NaCl..............................................................................................5.0g
Phenol Red (2% solution)..........................................................1.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except agarose, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 7.3 with 1N NaOH. Add agarose. Mix thoroughly. Gently
heat and bring to boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the isolation and cultivation of human mycoplasmas and ure-
aplasmas.