N plus C Medium

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

N plus C Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
Glucose.......................................................................................10.0g
Citric acid·H 2 O ...........................................................................4.04g
KH 2 PO 4 .........................................................................................2.0g
Yeast extract..................................................................................1.5g
CaCl 2 ·2H 2 O ..................................................................................0.6g
MgSO 4 ·7H 2 O................................................................................0.6g
FeCl 2 ·4H 2 O.................................................................................0.06g
ZnSO 4 ·7H 2 O.............................................................................0.034g
Hemin solution...........................................................................1.0mL
pH 4.6 ± 0.2 at 25°C
Hemin Solution:
Composition per 100.0mL:
NaOH............................................................................................1.0g
Hemin ....................................................................................250.0mg
Preparation of Hemin Solution: Add components to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except hemin solution, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 4.6. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Prior to inoculation, add 0.1mL of hemin solution per
100.0mL of medium.
Use: For the cultivation and maintenance of Physarum polycephalum.