N DeVogel Medium (Vogel N Medium)

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

N DeVogel Medium
(Vogel N Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................15.0g
KH 2 PO 4 ........................................................................................5.0g
Trisodium citrate·2H 2 O ................................................................3.0g
NH 4 NO 3 ........................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·H 2 O solution .................................................................20.0mL
Biotin solution ...........................................................................5.0mL
Trace elements solution.............................................................5.0mL
CaCl 2 ·H 2 O Solution:
Composition per 20.0mL:
CaCl 2 ·H 2 O....................................................................................0.1g
Preparation of CaCl 2 ·H 2 O Solution:  Add CaCl 2 ·H 2 O to dis-
tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.
Biotin Solution:
Composition per 100.0mL:
Biotin .........................................................................................5.0mg
Preparation of Biotin Solution:  Add biotin to 50% ethanol and
bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Store at 5°C.
Trace Elements Solution:
Composition per 100.0mL:
Citric acid·H 2 O.............................................................................5.0g
ZnSO 4 ·7H 2 O.................................................................................5.0g
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O .................................................................1.0g
CuSO 4 ·5H 2 O...............................................................................0.25g
H 3 BO 3 , anhydrous......................................................................0.05g
MnSO 4 ·H 2 O................................................................................0.05g
Na 2 MoO 4 ·2H 2 O..........................................................................0.05g
Preparation of Trace Elements Solutions: Add components
successively to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly after addition of each component. Filter
sterilize. Add 2–3.0mL of chloroform as a preservative. Store at 25°C.
Preparation of Medium: Add components, except biotin solution
and trace elements solution, to distilled/deionized water and bring vol-
ume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 5.0mL of sterile biotin solution and
5.0mL of sterile trace elements solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Neurospora crassa.