Nakayama Glucose Agar

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Nakayama Glucose Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Yeast extract................................................................................15.0g
Agar ............................................................................................10.0g
Glucose .......................................................................................10.0g
Peptone........................................................................................10.0g
Solution A................................................................................10.0mL
Solution B................................................................................10.0mL
Solution C..................................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Solution A:
Composition per 100.0mL:
K 2 HPO 4 .........................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution B:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................3.0g
MnSO 4 ·5H 2 O................................................................................0.1g
NaCl..............................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.01g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution C:
Composition per 100.0mL:
Trisodium citrate...........................................................................2.0g
FeSO 4 ·7H 2 O..................................................................................0.1g
Preparation of Solution C:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except solution A, so-
lution B, and solution C, to distilled/deionized water and bring volume
to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti-
cally add 10.0mL of sterile solution A, 10.0mL of sterile solution B,
and 1.0mL of sterile solution C. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Bacillus laevolacticus.