NAM Medium

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

NAM Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................15.0g
Agar............................................................................................15.0g
Papaic digest of soybean meal......................................................5.0g
NaCl..............................................................................................5.0g
Sheep blood, defibrinated........................................................50.0mL
Hemin solution.........................................................................10.0mL
N-Acetyl muramic acid (NAM) solution...................................1.0mL
pH 7.3 ± 0.2 at 25°C
Hemin Solution:
Composition per 100.0mL:
Hemin .......................................................................................0.050g
NaOH (1N solution)...................................................................1.0mL
Preparation of Hemin Solution: Add hemin to NaOH solution.
Mix thoroughly. Adjust volume to 100.0mL with distilled/deionized wa-
ter. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
N-Acetyl Muramic Acid (NAM) Solution:
Composition per 10.0mL:
N-Acetyl muramic acid..........................................................100.0mg
Preparation of N-Acetyl Muramic Acid (NAM) Solution:
Add N-acetyl muramic acid to distilled/deionized water and bring vol-
ume to 10.0mL. Filter sterilize.
Preparation of Medium: Add components, except sheep blood,
hemin solution, and N-acetyl muramic acid (NAM) solution, to dis-
tilled/deionized water and bring volume to 49.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 50.0mL of sterile sheep blood, 10.0mL of sterile hemin
solution, and 1.0mL of sterile N-acetyl muramic acid (NAM) solution.
Mix thoroughly. Aseptically and anaerobically distribute into sterile
tubes under a gas phase of 80% N 2  + 10% CO 2  + 10% H 2 .
Use: For the cultivation of Bacteroides forsythus.