NANAT Agar (Nalidixic Acid Novobiocin Actidione Tellurite Agar)

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

NANAT Agar
(Nalidixic Acid Novobiocin Actidione Tellurite Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................17.0g
Agar ............................................................................................15.0g
NaCl..............................................................................................5.0g
Tween™ 80...................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
Glucose .........................................................................................2.5g
Yeast extract..................................................................................1.0g
Tellurite solution......................................................................10.0mL
Antibiotic solution ...................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Tellurite Solution:
Composition per 100.0mL:
K 2 TeO 3 ........................................................................................0.05g
Preparation of Tellurite Solution: Add K 2 TeO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Antibiotic Solution:
Composition per 10.0mL:
Actidione (cycloheximide) .........................................................0.04g
Polymyxin B (optional) ..............................................................0.03g
Novobiocin................................................................................0.025g
Nalidixic acid..............................................................................0.02g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution: Potassium tellurite is toxic.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except tellurite solu-
tion and antibiotic solution, to distilled/deionized water and bring vol-
ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile tellurite solution and antibiotic solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Rhodococcus (Corynebacte-
rium) equi from animal feces, especially from horses and swine. The
addition of polymyxin B inhibits the growth of Pseudomonas aerugi-
nosa which may interfere with the isolation of Rhodococcus equi.