Natronobacteria Medium

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Natronobacteria Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................200.0g
Agar ............................................................................................20.0g
Yeast extract..................................................................................5.0g
Casamino acids.............................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
KCl................................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
Sodium glutamate.........................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.24g
CaSO 4 ·2H 2 O...............................................................................0.17g
Na 2 CO 3 solution....................................................................100.0mL
Trace elements solution SL-6 ....................................................1.0mL
pH 9.0 ± 0.2 at 25°C
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3  .........................................................................................5.0g
Preparation of Na 2 CO 3 Solution: Add Na 2 CO 3  to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 · 2H 2 O.................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Medium: Add components, except Na 2 CO 3 solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
Na 2 CO 3 solution. Mix thoroughly. Adjust pH to 9.0, if necessary. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Natronobacterium grego-
ryi, Natronobacterium magadii, Natronobacterium pharaonis, and
Natronococcus occultus.