Natronobacterium Medium

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Natronobacterium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................250.0g
Agar ............................................................................................20.0g
Casamino acids...........................................................................15.0g
Trisodium citrate·2H 2 O.................................................................3.0g
Glutamic acid................................................................................2.5g
MgSO 4 ·7H 2 O................................................................................2.5g
KCl................................................................................................2.0g
Na 2 CO 3  solution..................................................................... variable
pH 8.5 ± 0.2 at 25°C
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3  .........................................................................................5.0g
Preparation of Na 2 CO 3 Solution:  Add Na 2 CO 3  to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except salt and
Na 2 CO 3  solution, to distilled/deionized water and bring volume to
850.0mL. Mix thoroughly. Gently heat and bring to boiling. Add salt.
Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°–55°C. Adjust pH to 8.5 with sterile Na 2 CO 3
solution.
Use:  For the cultivation and maintenance of Natronobacterium mag-
adii, Natronobacterium pharaonis, and Natronococcus occultus.