NBY Medium (Nutrient Broth Yeast Extract Medium)

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

NBY Medium
(Nutrient Broth Yeast Extract Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Nutrient broth, dehydrated............................................................8.0g
Yeast extract..................................................................................2.0g
K 2 HPO 4 .........................................................................................2.0g
KH 2 PO 4 .........................................................................................0.5g
Glucose solution ......................................................................50.0mL
MgSO 4 ·7H 2 O (1M solution)......................................................1.0mL
Glucose Solution:
Composition per 50.0mL:
D- Glucose......................................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glu-
cose solution. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes.
Use:  For the cultivation and maintenance of Curtobacterium flaccum-
faciens and Pseudomonas syringae.