NBY Medium (Nutrient Broth Yeast Extract Medium) (ATCC Medium 763)

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

NBY Medium
(Nutrient Broth Yeast Extract Medium)
(ATCC Medium 763)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Nutrient broth................................................................................8.0g
Yeast extract..................................................................................2.0g
K 2 HPO 4 .........................................................................................2.0g
KH 2 PO 4 .........................................................................................0.5g
Glucose solution ......................................................................50.0mL
MgSO 4  solution........................................................................50.0mL
Glucose Solution:
Composition per 50.0mL:
D- Glucose......................................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril-
ize.
MgSO 4 Solution:
Composition per 50.0mL:
MgSO 4 ·7H 2 O..............................................................................0.25g
Preparation of MgSO 4 Solution: Add the solid MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 50.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Preparation of Medium: Add components, except glucose solu-
tion and MgSO 4  solution, to distilled/deionized water and bring vol-
ume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 25 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile glucose solution and MgSO 4  solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Bacillus sphaericus.