Neomycin Agar

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neomycin Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .........................................................................................6.0g
Pancreatic digest of casein............................................................4.0g
Yeast extract..................................................................................3.0g
Beef extract...................................................................................1.5g
Glucose.........................................................................................1.0g
Neomycin solution...................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Neomycin Solution:
Composition per 10.0mL:
Neomycin sulfate..........................................................................1.0g
Preparation of Neomycin Solution: Add neomycin sulfate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except neomycin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile neo-
mycin solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use:  For the cultivation and maintenance of Micrococcus luteus.