Neomycin Agar, Modified

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neomycin Agar, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .........................................................................................6.0g
Pancreatic digest of casein............................................................4.0g
Yeast extract..................................................................................3.0g
Beef extract...................................................................................1.5g
Glucose.........................................................................................1.0g
Methanol..................................................................................20.0mL
Neomycin solution...................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Neomycin Solution:
Composition per 10.0mL:
Neomycin sulfate..........................................................................1.0g
Preparation of Neomycin Solution: Add neomycin sulfate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except methanol and
neomycin solution, to distilled/deionized water and bring volume to
970.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Filter sterilize
methanol. To cooled, sterile basal medium, aseptically add sterile
methanol and sterile neomycin solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Bordetella bronchisep-
tica.