Neopeptone Glucose Rose Bengal Aureomycin ® Agar

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neopeptone Glucose Rose Bengal Aureomycin ® Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Neopeptone...................................................................................5.0g
Glucose .........................................................................................1.0g
Tetracycline solution..................................................................5.0mL
Rose Bengal solution.................................................................3.5mL
pH 6.5 ± 0.2 at 25°C
Tetracycline Solution:
Composition per 150.0mL:
Tetracycline...................................................................................1.0g
Preparation of Tetracycline Solution: Add tetracycline to dis-
tilled/deionized water and bring volume to 150.0mL. Mix thoroughly.
Filter sterilize.
Rose Bengal Solution:
Composition per 100.0mL:
Rose Bengal..................................................................................1.0g
Preparation of Rose Bengal Solution: Add Rose Bengal to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except tetracycline so-
lution, to distilled/deionized water and bring volume to 995.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL of
sterile tetracycline solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of a wide variety of fungal spe-
cies.