Neopeptone Infusion Agar

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neopeptone Infusion Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from..........................................................500.0g
Neopeptone.................................................................................20.0g
Agar ............................................................................................20.0g
NaCl..............................................................................................5.0g
Sheep blood, defibrinated........................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of a wide variety of fastidious microorgan-
isms.