Neurospora Medium

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neurospora Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Glucose.........................................................................................5.0g
Malt syrup, spray dried.................................................................5.0g
Sucrose..........................................................................................5.0g
Yeast extract..................................................................................2.5g
Vitamin solution.......................................................................10.0mL
Casein, hydrolyzed ....................................................................5.0mL
Vitamin Solution:
Composition per liter:
Ribonucleic acid, alkali hydrolyzed..............................................0.5g
Inositol..........................................................................................0.4g
Choline..........................................................................................0.2g
Nicotinamide.................................................................................0.2g
Pantothenic acid............................................................................0.2g
Thiamine.......................................................................................0.1g
p-Aminobenzoic acid..................................................................0.05g
Pyridoxine...................................................................................0.05g
Riboflavin ...................................................................................0.05g
Folic acid.....................................................................................4.0μg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile vitamin solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of Neurospora species on complete medium.