Neurospora Medium

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neurospora Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................15.0g
Ammonium tartrate.......................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
NH 4 NO 3 ........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 .............................................................................................0.1g
NaCl..............................................................................................0.1g
ZnCl 2 ..........................................................................................2.0mg
FeCl 3 ..........................................................................................0.2mg
Cu Cl 2 .........................................................................................0.1mg
MnCl 2 .......................................................................................0.02mg
Na 2 MoO 4 ·2H 2 O .......................................................................0.02mg
H 3 BO 3 ......................................................................................0.01mg
Biotin ..........................................................................................1.0μg
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Neurospora species on minimal medium.