Neurospora Minimal Medium

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neurospora Minimal Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................20.0g
Agar............................................................................................20.0g
KH 2 PO 4 .........................................................................................5.0g
Trisodium citrate·2H 2 O.................................................................2.5g
NH 4 NO 3 ........................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Sucrose-agar solution.............................................................200.0mL
Biotin solution ...........................................................................1.0mL
Trace elements solution.............................................................0.1mL
pH 5.8 ± 0.2 at 25°C
Sucrose-Agar Solution:
Composition per 200.0mL:
Sucrose........................................................................................20.0g
Agar............................................................................................20.0g
Preparation of Sucrose-Agar Solution:  Add components to dis-
tilled/deionized water and bring volume to 200.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C.
Biotin Solution:
Composition per 10.0mL:
Biotin........................................................................................50.0μg
Preparation of Biotin Solution:  Add biotin to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Trace Elements Solution:
Composition per 100.0mL
Citric acid·H 2 O .............................................................................5.0g
ZnSO 4 ·7H 2 O.................................................................................5.0g
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O..................................................................1.0g
CuSO 4 ·5H 2 O...............................................................................0.25g
MnSO 4 ·H 2 O................................................................................0.05g
H 3 BO 3 .........................................................................................0.05g
Na 2 MoO 4 ·2H 2 O..........................................................................0.05g
Preparation of Trace Elements Solution:  Add components one
at a time to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize. Add 1.0mL of chloroform as a preser-
vative. Store at room temperature.
Preparation of Medium: Add trisodium citrate·2H 2 O, KH 2 PO 4 ,
NH 4 NO 3 , MgSO 4 ·7H 2 O, and CaCl 2 ·2H 2 O to distilled/deionized water
and bring volume to 800.0mL. Make sure that one component is dis-
solved completely before adding the next one. This is conveniently
done in a Fernbach flask on a shaker. Mix thoroughly. Filter sterilize.
Warm solution to 45°–50°C. Add 200.0mL of sterile sucrose-agar so-
lution, 1.0mL of sterile biotin solution, and 0.1mL of sterile trace ele-
ments solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation and maintenance of Cochliobolus sativus,
Fusarium solani, Neurospora crassa, and Neurospora sitophila.