Nevskia Medium (DSMZ Medium 828)

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Nevskia Medium
(DSMZ Medium 828)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na-lactate....................................................................................0.56g
MgSO 4 ·7H 2 O..............................................................................0.05g
CaCl 2 ·2H 2 O...............................................................................0.014g
Potassium phosphate buffer, 1M, pH 7....................................27.5mL
Trace elements solution SL-9 ....................................................0.5mL
Seven vitamin solution...............................................................0.5mL
pH 7.1 ± 0.2 at 25°C
Trace Elements Solution SL-9:
Composition per liter:
Nitrilotriacetic acid.....................................................................12.8g
FeCl 2 ·4H 2 O...................................................................................3.5g
CoCl 2 ·6H 2 O ...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-9: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Adjust pH to 6.0. Sparge with 80% N 2 + 20% CO 2 . Autoclave
for 15 min at 15 psi pressure–121°C.
Seven Vitamin Solution:
Composition per liter:
Pyridoxine hydrochloride ......................................................300.0mg
Thiamine-HCl·2H 2 O..............................................................200.0mg
Nicotinic acid.........................................................................200.0mg
Vitamin B 12 ............................................................................100.0mg
Calcium pantothenate ............................................................100.0mg
p-Aminobenzoic acid...............................................................80.0mg
D (+)-Biotin...............................................................................20.0mg
Preparation of Seven Vitamin Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Sparge with 100% N 2 .
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except seven vitamin
solution, to distilled/deionized water and bring volume to 999.5mL.
Mix thoroughly. Adjust pH to 7.1. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to room temperature. Aseptically add 0.5mL seven
vitamin solution. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use:  For the cultivation of Nevskia ramosa.