New York City Medium

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

New York City Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NYC basal medium................................................................640.0mL
Horse blood cells ...................................................................200.0mL
Horse plasma, citrated............................................................120.0mL
Yeast dialysate..........................................................................25.0mL
Glucose solution ......................................................................10.0mL
Antibiotic VCNT solution .........................................................5.0mL
pH 7.4 ± 0.2 at 25°C
NYC Basal Medium:
Composition per 640.0mL:
Solution 1...............................................................................400.0mL
Solution 3...............................................................................200.0mL
Solution 2.................................................................................40.0mL
Preparation of NYC Basal Medium:  Combine solution 1, solu-
tion 2, and solution 3. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C.
Solution 1:
Composition per 400.0mL:
Agar............................................................................................20.0g
Preparation of Solution 1: Add agar to distilled/deionized water
and bring volume to 400.0mL. Mix thoroughly. Melt agar in autoclave
for 10 min at 0 psi pressure–100°C. Cool to 45°–50°C.
Solution 2:
Composition per 40.0mL:
Cornstarch.....................................................................................1.0g
Preparation of Solution 2: Add cornstarch to distilled/deionized
water and bring volume to 40.0mL. Mix thoroughly. Warm to 45°–
50°C.
Solution 3:
Composition per 200.0mL:
Proteose peptone No. 3...............................................................15.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
KH 2 PO 4 .........................................................................................1.0g
Preparation of Solution 3: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Cool to 45°–50°C.
Horse Blood Cells:
Composition per 200.0mL:
Horse blood cells, sedimented...................................................6.0mL
Preparation of Horse Blood Cells: Cow blood may be used in-
stead of horse blood but do not use sheep blood. Use cells freshly
packed by sedimentation. Do not pack by centrifugation. Aseptically
add 6.0mL of sedimented blood cells to 200.0mL of sterile distilled/de-
ionized water. Mix thoroughly.
Horse Plasma, Citrated:
Composition  per 6.0L:
Horse blood..........................................................................5400.0mL
Citrate solution.......................................................................600.0mL
Preparation of Horse Plasma, Citrated: Place 600.0mL of ster-
ile citrate solution into a receiving bottle. Draw horse blood to the 6.0L
mark. Allow cells to sediment out. Aseptically remove plasma.
Citrate Solution:
Composition per liter:
Sodium citrate...........................................................................150.0g
NaCl..........................................................................................81.13g
Preparation of Citrate Solution: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................5.0g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 10 min at 10 psi pressure–115°C. Cool to 45°–50°C.
Yeast Dialysate:
Composition per 2500.0mL:
Baker’s yeast, fresh...................................................................908.0g
Preparation of Yeast Dialysate: Add fresh baker’s yeast to
2500.0mL of distilled/deionized water. Mix thoroughly. Autoclave for
10 min at 15 psi pressure–121°C. Cool to 25°C. Put into dialysis tub-
ing. Dialyze against 2.0L of distilled/deionized water for 48 hr at 4°C.
Antibiotic VCNT Solution:
Composition per 5.0mL:
Colistin.......................................................................................7.5mg
Trimethorprim lactate ................................................................3.0mg
Vancomycin·HCl........................................................................2.0mg
Nystatin......................................................................................12.5U
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 5.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Have all solutions prepared and at 45°–
50°C. Aseptically combine components. Mix thoroughly. Pour into
sterile Petri dishes.
Use: For the isolation and cultivation of pathogenic Neisseria species.
Used as a transport medium for urogenital and other clinical specimens.
For the isolation and presumptive identification of Mycoplasmatales,
including large-colony species (Mycoplasma pneumoniae) and T–myco-
plasmas from urogenital specimens.