New York City Medium, Modified

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

New York City Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NYC basal medium................................................................840.0mL
α-Gamma horse serum (Flow Labs)......................................120.0mL
Yeast dialysate..........................................................................25.0mL
Glucose solution ......................................................................10.0mL
Antibiotic LCNT solution..........................................................5.0mL
pH 7.4 ± 0.2 at 25°C
NYC Basal Medium:
Composition per 840.0mL:
Horse blood..........................................................................5400.0mL
Solution 1...............................................................................600.0mL
Solution 3...............................................................................200.0mL
Solution 2.................................................................................40.0mL
Preparation of NYC Basal Medium: Combine solution 1, solu-
tion 2, and solution 3. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C.
Solution 1:
Composition per 600.0mL:
Agar ............................................................................................20.0g
Preparation of Solution 1: Add agar to distilled/deionized water
and bring volume to 600.0mL. Mix thoroughly. Melt agar in autoclave
for 10 min at 0 psi pressure–100°C. Cool to 45°–50°C.
Solution 2:
Composition per 40.0mL:
Cornstarch.....................................................................................1.0g
Preparation of Solution 2: Add cornstarch to distilled/deionized
water and bring volume to 40.0mL. Mix thoroughly. Warm to 45°–
50°C.
Solution 3:
Composition per 200.0mL:
Proteose peptone No. 3...............................................................15.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
KH 2 PO 4 .........................................................................................1.0g
Preparation of Solution 3: Add components to distilled/deionized
water and bring volume to 200.0mL. Mix thoroughly. Gently heat and
bring to boiling. Cool to 45°–50°C.
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for
10 min at 10 psi pressure–115°C. Cool to 45°–50°C.
Yeast Dialysate:
Composition per 2500.0mL:
Baker’s yeast, fresh...................................................................908.0g
Preparation of Yeast Dialysate: Add fresh Baker’s yeast to
2500.0mL of distilled/deionized water. Mix thoroughly. Autoclave for
10 min at 15 psi pressure–121°C. Cool to 25°C. Put into dialysis tub-
ing. Dialyze against 2.0L of distilled/deionized water for 48 hr at 4°C.
Antibiotic LCNT Solution:
Composition per 5.0mL:
Colistin.......................................................................................7.5mg
Lincomycin·HCl ........................................................................4.0mg
Trimethorprim lactate................................................................3.0mg
Nystatin...................................................................................... 12.5U
Preparation of Antibiotic LCNT Solution: Add the components
to distilled/deionized water and bring volume to 5.0mL. Mix thorough-
ly. Filter sterilize the solution.
Preparation of Medium: Have all solutions prepared and at 45°–
50°C. Aseptically combine components. Mix thoroughly. Pour into
sterile Petri dishes.
Use: For the isolation and cultivation of pathogenic Neisseria species.
Used as a transport medium for urogenital and other clinical specimens.
For the isolation and presumptive identification of Mycoplasmatales,
including large-colony species (Mycoplasma pneumoniae) and T–myco-
plasmas from urogenital specimens.