Niacin Assay HiVeg Medium

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Niacin Assay HiVeg Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................40.0g
Sodium acetate............................................................................20.0g
Plant acid hydrolysate, vitamin free ...........................................12.0g
KH 2 PO 4 .........................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
L -Cystine.......................................................................................0.4g
MgSO 4 ..........................................................................................0.4g
DL -Tryptophan..............................................................................0.2g
Adenine sulfate...........................................................................0.02g
FeSO 4 ..........................................................................................0.02g
Guanine hydrochloride ...............................................................0.02g
MnSO 4 ........................................................................................0.02g
Uracil..........................................................................................0.02g
NaCl............................................................................................0.02g
Pyridoxine hydrochloride..........................................................0.4mg
Riboflavin (Vitamin B 2 ) ............................................................0.4mg
Calcium pantothenate ................................................................0.2mg
Thiamine hydrochloride.............................................................0.2mg
p-Aminobenzoic acid (PABA)...................................................0.1mg
Biotin .......................................................................................0.08mg
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL
volumes. Add standard solution or test solutions to each tube. Adjust
the volume of each tube to 10.0mL with distilled/deionized water. Au-
toclave for 10 min at 15 psi pressure–121°C.
Use: For the microbiological assay of nicotinic acid or nicotinamide
(niacin) using Lactobacillus plantarum as the test organism.