Nickels and Leesment Agar, Modified

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nickels and Leesment Agar, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Part 1......................................................................................750.0mL
Part 2......................................................................................100.0mL
Part 3......................................................................................100.0mL
Part 4........................................................................................50.0mL
pH 6.65 ± 0.2 at 25°C
Part 1:
Composition per 750.0mL:
Pancreatic digest of casein..........................................................20.0g
Lactose........................................................................................10.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................4.0g
Gelatin...........................................................................................2.5g
Sodium citrate...............................................................................2.0g
Preparation of Part 1:  Add components to distilled/deionized wa-
ter and bring volume to 750.0mL. Mix thoroughly. Adjust pH to 6.65.
Part 2:
Composition per 500.0mL:
Nonfat dry milk...........................................................................50.0g
Preparation of Part 2: Add nonfat dry milk to distilled/deionized
water and bring volume to 500.0mL. Inoculate with Lactobacillus bul-
garicus. Incubate at 20°C for 24 hr. Centrifuge at 5000 rpm for 10 min
to separate the curd. Collect the supernatant solution. Autoclave for 15
min at 15 psi pressure–121°C. Store at 4°C.
Part 3:
Composition per 100.0mL:
Calcium citrate............................................................................13.3g
Carboxymethylcellulose...............................................................0.8g
Preparation of Part 3: Combine the calcium citrate and carboxy-
methylcellulose in a mortar and grind until a fine powder. Add powder
to 100.0mL of hot distilled/deionized water. Mix thoroughly. Filter
through cheesecloth.
Part 4:
Composition per 50.0mL:
Calcium lactate .............................................................................8.0g
Preparation of Part 4: Add calcium lactate to distilled/deionized
water and bring volume to 50.0mL. Mix thoroughly. Gently heat until
dissolved.
Preparation of Medium:  Prepare each of the four parts separately.
Autoclave each part for 15 min at 15 psi pressure–121°C. Aseptically
combine part 1, part 2, part 3, and part 4. Mix thoroughly. Pour into
sterile Petri dishes. Swirl flask while dispensing medium.
Use: For the isolation and cultivation of acid-producing microorgan-
isms from foods.