Nicotinic Acid Medium (DSMZ Medium 152)

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nicotinic Acid Medium
(DSMZ Medium 152)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................10.0g
Nicotinic acid................................................................................5.0g
Cysteine-HCl·H 2 O........................................................................0.5g
NaHCO 3 ........................................................................................0.4g
NaCl.........................................................................................80.0mg
KH 2 PO 4 ....................................................................................40.0mg
K 2 HPO 4 ....................................................................................40.0mg
CaCl 2 ·2H 2 O ...............................................................................8.0mg
MgSO 4 ·7H 2 O.............................................................................8.0mg
Distilled water......................................................................1000.0mL
pH 8.2 ± 0.2 at 25°C
Preparation of Medium:  Add nicotinic acid to distilled/deionized
water and bring volume to 1.0L. Gently heat and bring to boiling. Boil
with mixing until nicotinic acid is fully dissolved. Cool to 25°C. Add
remaining components. Readjust volume to 1.0L with distilled/deion-
ized water. Mix thoroughly. Adjust pH to 8.2. Distribute into tubes or
flasks under 100% nitrogen atmosphere. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the cultivation and maintenance of Eubacterium barkeri.