Nitrate Liquid Medium

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Liquid Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................500.0mL
Solution B..............................................................................250.0mL
Solution C..............................................................................250.0mL
Solution A:
Composition per 500.0mL:
Mannitol......................................................................................10.0g
KNO 3 ............................................................................................0.6g
Na 2 HPO 4 ·12H 2 O.........................................................................0.45g
Na 2 SO 4 ........................................................................................0.03g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C.
Solution B:
Composition per 250.0mL:
MgSO 4 ·7H 2 O..............................................................................0.12g
CaCl 2 ·6H 2 O...................................................................................0.1g
FeCl 3 ·6H 2 O.................................................................................0.01g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C.
Solution C:
Composition per 250.0mL:
Calcium pantothenate ................................................................0.5mg
Thiamine·HCl ............................................................................0.1mg
Biotin ..........................................................................................0.5μg
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine 500.0mL of cooled,
sterile solution A, 250.0mL of cooled, sterile solution B, and 250.0mL
of sterile solution C. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the isolation and cultivation of Rhizobium species.