Nitrate Methanol Medium

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Methanol Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaNO 3 ...........................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
NaCl..............................................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.02g
Na 2 MoO 4 ·H 2 O ...........................................................................1.0mg
Riboflavin ..................................................................................0.2mg
Calcium pantothenate ................................................................0.2mg
Pyridoxine·HCl..........................................................................0.2mg
Nicotinic acid.............................................................................0.2mg
Thiamine·HCl ............................................................................0.1mg
p-Aminobenzoic acid.................................................................0.1mg
Biotin.......................................................................................0.01mg
Methanol..................................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Filter sterilize methanol. Aseptically add sterile methanol to cooled
sterile medium. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use:  For the cultivation and maintenance of Methylobacterium rhodi-
num.