Nitrate Mineral Salts Medium (NMS Medium)

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Mineral Salts Medium
(NMS Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar...................................................................................12.5g
MgSO 4 ·7H 2 O................................................................................1.0g
KNO 3 ............................................................................................1.0g
Na 2 HPO 4 ·12H 2 O.......................................................................0.717g
KH 2 PO 4 .....................................................................................0.272g
CaCl 2 ·6H 2 O ..................................................................................0.2g
Ferric ammonium EDTA...........................................................4.0mg
Trace elements solution.............................................................0.5mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Disodium EDTA...........................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
H 3 BO 3 .........................................................................................0.03g
CoCl 2 ·6H 2 O................................................................................0.02g
ZnSO 4 ·7H 2 O...............................................................................0.01g
MnCl 2 ·4H 2 O ..............................................................................3.0mg
Na 2 MoO 4 ·2H 2 O.........................................................................3.0mg
NiCl 2 ·6H 2 O................................................................................2.0mg
CaCl 2 ·2H 2 O ...............................................................................1.0mg
Preparation of Trace Elements Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use:  For the cultivation and maintenance of Methylobacterium spe-
cies, Methylococcus capsulatus, Methylomonas agile, and Methylomo-
nas methanica.