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Nitrate Mineral Salts Medium with Methanol (NMS Medium with Methanol)
[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]
Nitrate Mineral Salts Medium with Methanol
(NMS Medium with Methanol)
MgSO 4 ·7H 2 O................................................................................1.0g
KNO 3 ............................................................................................1.0g
Na 2 HPO 4 ·12H 2 O.......................................................................0.717g
KH 2 PO 4 .....................................................................................0.272g
CaCl 2 ·6H 2 O ..................................................................................0.2g
Ferric ammonium EDTA...........................................................4.0mg
Trace elements solution .............................................................0.5mL
Methanol....................................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Disodium EDTA ...........................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
H 3 BO 3 .........................................................................................0.03g
CoCl 2 ·6H 2 O ................................................................................0.02g
ZnSO 4 ·7H 2 O...............................................................................0.01g
MnCl 2 ·4H 2 O...............................................................................3.0mg
Na 2 MoO 4 ·2H 2 O .........................................................................3.0mg
NiCl 2 ·6H 2 O................................................................................2.0mg
CaCl 2 ·2H 2 O................................................................................1.0mg
Preparation of Trace Elements Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 999.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Adjust pH to 6.8. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Filter sterilize methanol. Aseptically add sterile methanol
to cooled sterile medium. Mix thoroughly. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Methylobacterium fuji-
sawaense, Methylobacterium species, and Methylomonas clara.
(NMS Medium with Methanol)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Noble agar...................................................................................12.5gMgSO 4 ·7H 2 O................................................................................1.0g
KNO 3 ............................................................................................1.0g
Na 2 HPO 4 ·12H 2 O.......................................................................0.717g
KH 2 PO 4 .....................................................................................0.272g
CaCl 2 ·6H 2 O ..................................................................................0.2g
Ferric ammonium EDTA...........................................................4.0mg
Trace elements solution .............................................................0.5mL
Methanol....................................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Disodium EDTA ...........................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
H 3 BO 3 .........................................................................................0.03g
CoCl 2 ·6H 2 O ................................................................................0.02g
ZnSO 4 ·7H 2 O...............................................................................0.01g
MnCl 2 ·4H 2 O...............................................................................3.0mg
Na 2 MoO 4 ·2H 2 O .........................................................................3.0mg
NiCl 2 ·6H 2 O................................................................................2.0mg
CaCl 2 ·2H 2 O................................................................................1.0mg
Preparation of Trace Elements Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 999.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Adjust pH to 6.8. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Filter sterilize methanol. Aseptically add sterile methanol
to cooled sterile medium. Mix thoroughly. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Methylobacterium fuji-
sawaense, Methylobacterium species, and Methylomonas clara.
