Nutrient Agar pH 6.8, HiVeg with Blood

[所属分类:培养基配方] [发布时间:2021-6-22] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar pH 6.8, HiVeg with Blood

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Plant peptone.................................................................................5.0g
Plant extract ..................................................................................3.0g
Sheep blood, defibrinated........................................................50.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium, without blood, is available as a premixed pow-
der from HiMedia.
Preparation of Medium:  Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Heat with frequent agitation and boil for 1 min to completely dis-
solve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix
thoroughly and pour into sterile Petri dishes.
Use:  For the cultivation of streptococci and other fastidious microor-
ganisms.