Nutrient Agar pH 6.8 with Horse Blood (LMG Medium 234)

[所属分类:培养基配方] [发布时间:2021-6-22] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar pH 6.8 with Horse Blood
(LMG Medium 234)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone......................................................................................... 5.0g
NaCl..............................................................................................5.0g
Na 2 HPO 4 ·12 H 2 O........................................................................2.39g
Yeast extract..................................................................................2.0g
Lab-Lemco beef extract................................................................1.0g
KH 2 PO 4 .......................................................................................0.45g
Horse blood, sterile defibrinated..............................................50.0mL
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add 50.0mL sterile horse blood. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Neisseria weaveri.